42
ICCS 2011
P6
Evaluation of Lymphocyte Subset Enumeration in
Transplant Patients Using a Novel Flow Cytometer
That Integrates Sample Preparation and Analysis
in One System
Raquel Cabana
1
, Alexandra Amador
2
, Michael Brochu
Jr
1
, Phillip Ruiz
2
1
Blue Ocean Biomedical, Pembroke Pines, FL, USA,
2
Immune Monitoring Laboratory, University of Miami
Miller School of Medicine, Miami, FL, USA
The objective of the study was to compare the
process from sample collection to fnal result
when enumerating lymphocyte subsets by a novel
versus conventional fow cytometry technology.
The innovative system, the Blue Ocean™
CR300, a fully integrated cell analyzer which
automatically processes barcoded primary patient
tubes and performs sample preparation and
analysis simultaneously in one instrument without
operator intervention was compared to the manual
conventional clinical cytometry system. The study was
performed in the Immune Monitoring Laboratory at the
University of Miami. 171 samples from patients in an
organ transplant program were run in parallel using a
CR300 System and BD FACSCalibur™. The samples
were 95% from post transplant patients within 3 years
of transplantation. The mean age of patients was
49 years old with different solid organ transplant.
Percentages and absolute counts were obtained for
lymphocyte subpopulations in peripheral blood. Blue
Ocean panels LSA-1(CD45/CD4/CD8/CD3) and LSA-
2 (CD45/CD56+16/CD19/CD3) were compared with
BD Multitest 4-color panels. Absolute counts were
obtained by single platform (volumetric) on the CR300
and dual platform on the FACSCaliburs. The results
show strong correlation for percentages and absolute
counts for all the lymphocyte subpopulations tested.
Workfow improvements were signifcant, specifcally
in reducing user intervention and minimizing both
the number and skill level of required fow operators.
In addition, potential sample identifcation errors
were virtually eliminated. Finally, turnaround time
was reduced while maximizing sample throughput.
Although this study focused on lymphocyte subset
analysis, the CR 300 System is well-suited for other
applications that require repetitive testing.
POSTER ABSTRACTS